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Tung Bui

PI: Professor Richard O'Kennedy

"An elevation of cardiac troponin T (cTnT), a cardiac muscle protein, is indicative of cardiac damage. Thus, cTnT is used as a biomarker for early stage diagnosis of cardiovascular disease. My project focuses on the construction of anti-cTnT antigen binding fragments (Fab) library and selection of antibodies with high affinity to cTnT. Antibody variable domain genes are amplified by PCR from chicken spleen and bone marrow cDNA for a large and diverse antibody repertoire library. The whole Fab gene is genetically constructed by Splice by Overlap Extension PCR (SOE PCR), cloned into E. Coli, and subsequently displayed on bacteriophage. Rounds of Phage Display Biopanning are performed to enrich for positive antibodies. Selected antibodies are characterized by Enzyme Linked ImmunoSorbent Assay (ELISA). Potential antibodies with high affinity to cTnT can be used to incorporate into high sensitivity diagnostic devices that can detect small amounts of cTnT "

Tung is a 3rd year Biochemistry student at York University, Canada.

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	Tung Bui PI: Professor Richard O'Kennedy "An elevation of cardiac troponin T (cTnT), a cardiac muscle protein, is indicative of cardiac damage. Thus, cTnT is used as a biomarker for early stage diagnosis of cardiovascular disease. My project focuses on the construction of anti-cTnT antigen binding fragments (Fab) library and selection of antibodies with high affinity to cTnT. Antibody variable domain genes are amplified by PCR from chicken spleen and bone marrow cDNA for a large and diverse antibody repertoire library. The whole Fab gene is genetically constructed by Splice by Overlap Extension PCR (SOE PCR), cloned into E. Coli, and subsequently displayed on bacteriophage. Rounds of Phage Display Biopanning are performed to enrich for positive antibodies. Selected antibodies are characterized by Enzyme Linked ImmunoSorbent Assay (ELISA). Potential antibodies with high affinity to cTnT can be used to incorporate into high sensitivity diagnostic devices that can detect small amounts of cTnT " Tung is a 3rd year Biochemistry student at York University, Canada. next